georesГеоресурсыGeoresources1608-50431608-5078Georesursy LLC10.18599/grs.2022.3.18geores-125Research ArticleГЕОЭКОЛОГИЧЕСКИЕ ИССЛЕДОВАНИЯGEOECOLOGICAL STUDIESВлияние углеродных сорбентов на потенциальную способность почв к самоочищению от нефтяного загрязненияInfluence of carbon sorbents on the potential ability of soils to self-cleaning from petroleum pollutionСмирноваЕ. В.SmirnovaE. V.

Елена Васильевна Смирнова – кандидат биол. наук, заведующий кафедрой почвоведения Института экологии и природопользования

420008, Казань, ул. Кремлевская, д.18

Elena V. Smirnova – Cand. Sci. (Biology), Head of the Department of Soil Science

18, Kremlevskaya st., Kazan, 420008

elenavsmirnova@mail.ruОкуневР. В.OkunevR. V.

Родион Владимирович Окунев – кандидат биол. наук, доцент кафедры почвоведения Института экологии и природопользования

420008, Казань, ул. Кремлевская, д.18

Rodion V. Okunev – Cand. Sci. (Biology), Associate Professor, Department of Soil Science

18, Kremlevskaya st., Kazan, 420008

ГиниятуллинК. Г.GiniyatullinK. G.

Камиль Гашикович Гиниятуллин – кандидат биол. наук, доцент кафедры почвоведения Института экологии и природопользования

420008, Казань, ул. Кремлевская, д.18

Kamil G. Giniyatullin – Cand. Sci. (Biology), Associate Professor, Department of Soil Science

18, Kremlevskaya st., Kazan, 420008

Казанский (Приволжский) федеральный университетРоссияKazan Federal UniversityRussian Federation202213042024243210218Copyright © Смирнова Е.В., Окунев Р.В., Гиниятуллин К.Г., 20242024Смирнова Е.В., Окунев Р.В., Гиниятуллин К.Г.Smirnova E.V., Okunev R.V., Giniyatullin K.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.geors.ru/jour/article/view/125

В лабораторном эксперименте изучали способность почв к самоочищению при сильном нефтяном загрязнении и влияние на данный процесс биоуглей и шунгитов. Инкубирование почв, загрязненных нефтью, без добавления сорбентов при постоянной оптимальной влажности и температуре в течение 28 суток обеспечило снижение остаточного содержания нефтепродуктов (НП) лишь на 8%. Добавление биоугля и шунгита в дозе 2,5% позволило снизить содержание НП при постоянных условиях инкубации до 48,8% и 38%, соответственно. Показано, что проведение инкубации нефтезагрязненных почв в режиме переменной влажности и температуры без добавления сорбентов позволяет снизить остаточное содержание НП за 28 дней эксперимента на 32%. В процессе исследования отработаны методы определения субстрат-индуцированного дыхания (СИД) в различных режимах инкубации. Сильное загрязнение почвы нефтью привело к существенному уменьшению в начальный период интенсивности СИД с 12,8 C-CO2 мкг/г ч до 8,6 C-CO2 мкг/г ч, которое нормализовалось на 14-й день проведения опыта. Показано, что внесение биуглей (в меньшей степени шунгитов) в почву, загрязненную нефтью, обеспечивает поддержание СИД на необходимом уровне и увеличивает потенциальную способность почв к самоочищению. В работе обсуждаются возможности увеличения потенциальной способности почв к самоочищению при сильном загрязнении нефтью и НП.

In a laboratory experiment, the ability of the soil to self-cleaning under heavy petroleum pollution and the effect of biochars and shungites on the cleaning process were studied. Incubation of contaminated soils for 28 days at a constant humidity and temperature without addition of sorbents led to a decrease in the residual content of petroleum products by only 8%. The addition of biochar and shungite at a dose of 2.5% made it possible to reduce the content of petroleum under constant incubation conditions to 48.8% and 38%, respectively. It was shown that the incubation of oil-contaminated soils in the regime of variable humidity and temperature without the addition of sorbents makes it possible to reduce the content of petroleum by 32% over 28 days of the experiment. In the course of the study, methods were developed for determining substrate-induced respiration (SIR) in various incubation modes. Soil contamination with petroleum led to a significant decrease of SIR in the initial period of incubation from 12.8 C-CO2 µg/g h to 8.6 C-CO2 µg/g h, which returned to normal on the 14th day of the experiment. It has been shown that the introduction of biochars (to a lesser extent schungites) into oil-contaminated soils ensures the maintenance of SIR at the required level and increases the potential capacity of soils for self-purification. The paper discusses the possibilities of increasing the potential capacity of soils for self-cleaning under heavy oil pollution.

загрязнение почв нефтьюбиоугльшунгитыспособность почв к самоочищениюoil contamination of soilsbiocharshungitesself-cleaning capacity of soilsРабота выполнена при финансовой поддержке Российского научного фонда, проект № 22-24-00242.This work was supported by the Russian Science Foundation, research project № 22-24-00242ReferencesАнаньева Н.Д., Благодатская Е.В., Демкина Т.С. (1997). Влияние высушивания-увлажнения и замораживания-оттаивания на устойчивость микробных сообществ почвы. Почвоведение, 9, с. 1132-1137.Akbarzadeh K., Hammami A., Kharrat A., et al. (2007). Asphaltenes – problematic but rich in potential. Oilfield Review, 19(2), pp. 22-43.Березкин В.И. (2005). О сажевой модели происхождения Карельских шунгитов. Геология и геофизика, 46(10), с. 1093-1101.Ananyeva N.D., Blagodatskaya Е.V., Orlinsky D.B., Macchina Т.N. (1993). Methodical aspects of determining the rate of substrate-induced respiration of soil microorganisms. Soil Science, 11, pp. 72-77.Гилязов М.Ю. (1980). Изменение некоторых агрохимических свойств выщелоченного чернозема при загрязнении его нефтью. Агрохимия, 12, c. 72-75.Anan’eva N.D., Blagodatskaya E.V., Demkina T.S. (1997). The Effect of Drying-Moistening and Freezing-Thawing on Soil Microbial Communities Resilience. Eurasian Soil Science, 30(9), pp. 1010-1014.Глазовская М.А., Пиковский Ю.И. (1980). Скорости самоочищения почв от нефти в различных природных зонах. Природа, 5, c. 118-119.Atlas R.M., Stoeckel D.M., Faith S.A. et al. (2015). Oil Biodegradation and Oil-Degrading Microbial Populations in Marsh Sediments Impacted by Oil from the Deepwater Horizon Well Blowout. Environmental Science and Technology, 49, pp. 8356-8366. https://doi.org/10.1021/acs.est.5b00413Гордеев А.С., Гайнуллина Л.А., Шинкарев А.А. (2014). Липиды пахотных почв, систематически загрязняемых поверхностным склоновым стоком с территории объектов нефтедобычи. Ученые записки Казанского университета. Серия Естественные науки, 156(3), с. 76-86.Berezkin V.I. (2005). A soot model for the genesis of karelian shungites. Geologiya I Geofizika, 46(10), pp. 1093-1101. (In Russ.)Дейнес Ю.Е., Первунина А.В. (2019). Генезис высокоуглеродистых шунгитоносных пород – обзор. Труды Ферсмановской научной сессии ГИ КНЦ РАН, 16, с. 136-140. https://doi.org/10.31241/FNS.2019.16.028Bilias F., Nikoli T., Kalderis D., Gasparatos, D. (2021). Towards a Soil Remediation Strategy Using Biochar: Effects on Soil Chemical Properties and Bioavailability of Potentially Toxic Elements. Toxics, 9(8), 184. https://doi.org/10.3390/toxics9080184Исмаилов Н.М. (1988). Микробиология и ферментативная активность нефтезагрязненных почв. Восстановление нефтезагрязненных почвенных экосистем. Москва: Наука, с. 42–56.Blagodatskaya E., Kuzyakov Y. (2013). Active microorganisms in soil: critical review of estimation criteria and approaches. Soil Biology and Biochemistry, 67, pp. 192-211. https://doi.org/10.1016/j.soilbio.2013.08.024Киреева Н.А., Галимзянова Н.Ф. (1995). Влияние загрязнения почв нефтью и нефтепродуктами на численность и видовой состав микромицетов. Почвоведение, 2, с. 211-216.Chaineau C.-H., Yepremian C., Vidalie J. et al. (2003). Bioremediation of a Crude Oil-Polluted Soil: Biodegradation, Leaching and Toxicity Assessments. Water Air and Soil Pollution, 144, pp. 419-440. https://doi.org/10.1023/A:1022935600698Козловский Е.А. (1991). Горная энциклопедия. М: Яшма, 5, 451 с.Chen M., Xu P., Zeng G., et al. (2015). Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons, petroleum, pesticides, chlorophenols and heavy metals by composting: applications, microbes and future research needs. Biotechnology Advances, 33, pp. 745–755. https://doi.org/10.1016/j.biotechadv.2015.05.003Малыхина Л.В., Шайдуллина И.А., Антонов Н.А., Сибгатова Д.И., Яппаров А.Х., Дегтярева И.А., Латыпова В.З., Гадиева Э.Ш. (2016). Применение новых биотехнологий при рекультивации черноземов со смешанным типом загрязнения. Георесурсы, 18(2), c. 138-144. DOI: 10.18599/grs.18.2.12Deines Yu.Е., Pervunina А.V. (2019). The genesis of high-carbon shungite rocks – review. Trudy Fersmanovskoy nauchnoy sessii GI KNTs RAN, 16, pp. 136–140. (In Russ.) https://doi.org/10.31241/FNS.2019.16.028Мусина У.Ш., Васичкин А.С. (2014). Обзор способов утилизации нефтеотходов и технологии их утилизации. Вестник КазГАСА, 2(52), c. 133-141.El-Sheekh M.M., Hamouda R.A. (2014). Biodegradation of crude oil by some cyanobacteria under heterotrophic conditions. Desalination and Water Treatment, 52, pp. 1448-1454. https://doi.org/10.1080/19443994.2013.794008Петухова Г.А., Кулькова Т.А. (2021). Перспективы применения шунгита как сорбента формальдегида в композитных материалах. Сорбционные и хроматографические процессы, 21(1), c. 100-110.Forrester S., Janik L., McLaughlin M. et al. (2013). Total Petroleum Hydrocarbon Concentration Prediction in Soils Using Diffuse Reflectance Infrared Spectroscopy. Soil Science Society of America Journal, 77, pp. 450-460. https://doi.org/10.2136/sssaj2012.0201Смирнова Е.В., Гиниятуллин К.Г., Валеева А.А., Ваганова Е.С. (2018). Пироугли как перспективные почвенные мелиоранты: оценка содержания и спектральные свойства их липидных фракций. Учен. зап. Казан. ун-та. Сер. Естеств. науки, 160(2), с. 259–275.Friend D.J. (1996). Remediation of Petroleum-contaminated Soils. Washington: National Academy Press, 580 p.Солнцева Н.П. (2001). Добыча нефти и геохимия природных ландшафтов. М: Изд-во МГУ, 376 с.Gilyazov M.Yu. (1980). Changes in some agrochemical properties of leached chernozem when it is contaminated with oil. Agrokhimiya, 12, pp. 72-75. (In Russ.)Akbarzadeh K., Hammami A., Kharrat A., et al. (2007). Asphaltenes – problematic but rich in potential. Oilfield Review, 19(2), pp. 22-43.Glazovskaya M.A., Pikovskiy Yu.I. (1980). Rates of self-purification of soils from oil in various natural zones. Priroda, 5, pp. 118-119. (In Russ.)Ananyeva N.D., Blagodatskaya Е.V., Orlinsky D.B., Macchina Т.N. (1993). Methodical aspects of determining the rate of substrate-induced respiration of soil microorganisms. Soil Science, 11, pp. 72-77.Head I.M., Jones D.M., Röling W.F. (2006). Marine microorganisms make a meal of oil. Nature Reviews Microbiology, 4, p. 173. https://doi.org/10.1038/nrmicro1348Atlas R.M., Stoeckel D.M., Faith S.A. et al. (2015). Oil Biodegradation and Oil-Degrading Microbial Populations in Marsh Sediments Impacted by Oil from the Deepwater Horizon Well Blowout. Environmental Science and Technology, 49, pp. 8356-8366. https://doi.org/10.1021/acs.est.5b00413Hou N., Zhang N., Jia T. et al. (2018). Biodegradation of phenanthrene by biodemulsifier-producing strain Achromobacter sp. LH-1 and the study on its metabolisms and fermentation kinetics. Ecotoxicology and Environmental Safety, 163, pp. 205–214. https://doi.org/10.1016/j.ecoenv.2018.07.064Bilias F., Nikoli T., Kalderis D., Gasparatos, D. (2021). Towards a Soil Remediation Strategy Using Biochar: Effects on Soil Chemical Properties and Bioavailability of Potentially Toxic Elements. Toxics, 9(8), 184. https://doi.org/10.3390/toxics9080184Gordeev A.S., Gainullina L.A., Shinkarev A.A. (2014). Lipids in arable soils exposed to regular pollution by overland runoff from oil-production sites. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, 156(3), pp. 76-86. (In Russ.)Blagodatskaya E., Kuzyakov Y. (2013). Active microorganisms in soil: critical review of estimation criteria and approaches. Soil Biology and Biochemistry, 67, pp. 192-211. https://doi.org/10.1016/j.soilbio.2013.08.024Ismailov N.M. (1988). Microbiology and enzymatic activity of oilcontaminated soils. Restoration of oil-contaminated soil ecosystems. Moscow: Nauka, pp. 42-56. (In Russ.)Chaineau C.-H., Yepremian C., Vidalie J. et al. (2003). Bioremediation of a Crude Oil-Polluted Soil: Biodegradation, Leaching and Toxicity Assessments. Water Air and Soil Pollution, 144, pp. 419-440. https://doi.org/10.1023/A:1022935600698Jurgelane I., Locs J. (2021). Shungite application for treatment of drinking water – is it the right choice? Journal of Water and Health, 19, pp. 89-96. https://doi.org/10.2166/wh.2020.139Chen M., Xu P., Zeng G., et al. (2015). Bioremediation of soils contaminated with polycyclic aromatic hydrocarbons, petroleum, pesticides, chlorophenols and heavy metals by composting: applications, microbes and future research needs. Biotechnology Advances, 33, pp. 745–755. https://doi.org/10.1016/j.biotechadv.2015.05.003Jurgelāne I., Ločs J. (2021). Shungite Application for Treatment of Drinking Water – is It the Right Choice? Journal of Water and Health, 19(1), 89 p. https://doi.org/10.2166/wh.2020.139El-Sheekh M.M., Hamouda R.A. (2014). Biodegradation of crude oil by some cyanobacteria under heterotrophic conditions. Desalination and Water Treatment, 52, pp. 1448-1454. https://doi.org/10.1080/19443994.2013.794008Kireeva N.A., Galimzyanova N.F. (1995). Influence of soil pollution by oil and oil products on the abundance and species composition of micromycetes. Pochvovedenie, 2, pp. 211-216. (In Russ.)Forrester S., Janik L., McLaughlin M. et al. (2013). Total Petroleum Hydrocarbon Concentration Prediction in Soils Using Diffuse Reflectance Infrared Spectroscopy. Soil Science Society of America Journal, 77, pp. 450-460. https://doi.org/10.2136/sssaj2012.0201Kloss S., Zehetner F., Wimmer B. et al. (2014). Biochar application to temperate soils: Effects on soil fertility and crop growth under greenhouse conditions. Journal of Plant Nutrition and Soil Science, 177(1), pp. 3–15.Friend D.J. (1996). Remediation of Petroleum-contaminated Soils. Washington: National Academy Press, 580 p.Kwiecińska B., Pusz S., Krzesińska M., Pilawa B. (2007). Physical properties of shungite. International Journal of Coal Geology, 71, pp. 455-461. https://doi.org/10.1016/j.coal.2006.05.008Head I.M., Jones D.M., Röling W.F. (2006). Marine microorganisms make a meal of oil. Nature Reviews Microbiology, 4, p. 173. https://doi.org/10.1038/nrmicro1348Kovalev I.V., Kovaleva N.O. et al. (2008). Biochemistry of lignin in soils of periodic excessive moistening (from the example of agrogray soils in Opolie Landscapes of the Russian Plain). Eurasian Soil Science, 41(10), pp. 1066-1076. https://doi.org/10.1134/S1064229308100086Hou N., Zhang N., Jia T. et al. (2018). Biodegradation of phenanthrene by biodemulsifier-producing strain Achromobacter sp. LH-1 and the study on its metabolisms and fermentation kinetics. Ecotoxicology and Environmental Safety, 163, pp. 205–214. https://doi.org/10.1016/j.ecoenv.2018.07.064Kovalev I.V., Semenov V.M. et al. (2021). Estimation of the biogenicity and bioactivity of gleyed agrogray nondrained and drained soils. Eurasian Soil Science, 54(7), pp. 1059-1067. https://doi.org/10.1134/S1064229321070073Jurgelane I., Locs J. (2021). Shungite application for treatment of drinking water – is it the right choice? Journal of Water and Health, 19, pp. 89-96. https://doi.org/10.2166/wh.2020.139Labud V., Garcia C., Hernandez T. (2007). Effect of hydrocarbon pollution on the microbial properties of a sandy and a clay soil. Chemosphere, 66, pp. 1863-1871. https://doi.org/10.1016/j.chemosphere.2006.08.021Jurgelāne I., Ločs J. (2021). Shungite Application for Treatment of Drinking Water – is It the Right Choice? Journal of Water and Health, 19(1), 89 p. https://doi.org/10.2166/wh.2020.139Lacalle R.G., Becerril J.M., Garbisu C. (2020). Biological Methods of Polluted Soil Remediation for an Effective Economically-Optimal Recovery of Soil Health and Ecosystem Services. Journal of Environmental Science and Public Health, 4(2), pp. 112-133. https://doi.org/10.23986/afsci.8155Kloss S., Zehetner F., Wimmer B. et al. (2014). Biochar application to temperate soils: Effects on soil fertility and crop growth under greenhouse conditions. Journal of Plant Nutrition and Soil Science, 177(1), pp. 3–15.Lehmann J., Joseph S. (2009). Biochar for Environmental Management: Science and Technology. London: Earthscan, 416 р.Kwiecińska B., Pusz S., Krzesińska M., Pilawa B. (2007). Physical properties of shungite. International Journal of Coal Geology, 71, pp. 455-461. https://doi.org/10.1016/j.coal.2006.05.008Leon, V., Kumar, M. (2005). Biological upgrading of heavy crude oil. Biotechnology and Bioprocess Engineering, 10, pp. 471-481. https://doi.org/10.1007/BF02932281Kovalev I.V., Kovaleva N.O. et al. (2008). Biochemistry of lignin in soils of periodic excessive moistening (from the example of agrogray soils in Opolie Landscapes of the Russian Plain). Eurasian Soil Science, 41(10), pp. 1066-1076. https://doi.org/10.1134/S1064229308100086Ma Y.L., Lu W., Wan L.L., Luo N. (2015). Elucidation of fluoranthene degradative characteristics in a newly isolated Achromobacter xylosoxidans DN002. Applied Biochemistry and Biotechnology, 175, pp. 1294-1305. https://doi.org/10.1007/s12010-014-1347-7Kovalev I.V., Semenov V.M. et al. (2021). Estimation of the biogenicity and bioactivity of gleyed agrogray nondrained and drained soils. Eurasian Soil Science, 54(7), pp. 1059-1067. https://doi.org/10.1134/S1064229321070073Malykhina L.V., Shaydullina I.A., Antonov N.A. et al. (2016). Application of New Biotechnologies in the Remediation of Black Soil with Mixed Pollution. Georesursy = Georesources, 18(2), pp. 138-144. https://doi.org/10.18599/grs.18.2.12Labud V., Garcia C., Hernandez T. (2007). Effect of hydrocarbon pollution on the microbial properties of a sandy and a clay soil. Chemosphere, 66, pp. 1863-1871. https://doi.org/10.1016/j.chemosphere.2006.08.021Mambwe M., Kalebaila K.K., Johnson T. (2021). Remediation technologies for oil contaminated soil. Global Journal of Environmental Science and Management, 7(3), pp. 1-20. https://doi.org/10.22034/gjesm.2021.3.09Lacalle R.G., Becerril J.M., Garbisu C. (2020). Biological Methods of Polluted Soil Remediation for an Effective Economically-Optimal Recovery of Soil Health and Ecosystem Services. Journal of Environmental Science and Public Health, 4(2), pp. 112-133. https://doi.org/10.23986/afsci.8155Musina U.Sh., Vasichkin A.S. (2014). Overview of oil waste disposal methods and technologies for their disposal. Vestnik KazGASA, 2(52), pp.133-141. (In Russ.)Lehmann J., Joseph S. (2009). Biochar for Environmental Management: Science and Technology. London: Earthscan, 416 р.Okunev R., Smirnova E., Giniyatullin K. (2019). Study of the effect of labile organic matter removal from pyrochars on the substrate-induced respiration. SGEM, 19(3.2), pp. 459-465.Leon, V., Kumar, M. (2005). Biological upgrading of heavy crude oil. Biotechnology and Bioprocess Engineering, 10, pp. 471-481. https://doi.org/10.1007/BF02932281Pineda-Flores G., Boll-Arguello G., Lira-Galeana C., Mesta-Howard A.M. (2004). A microbial consortium isolated from a crude oil sample that uses asphaltenes as a carbon and energy source. Biodegradation, 15(3), pp. 145-151. https://doi.org/10.1023/b:biod.0000026476.03744.bbMa Y.L., Lu W., Wan L.L., Luo N. (2015). Elucidation of fluoranthene degradative characteristics in a newly isolated Achromobacter xylosoxidans DN002. Applied Biochemistry and Biotechnology, 175, pp. 1294-1305. https://doi.org/10.1007/s12010-014-1347-7Petukhova G. A., & Kulkova T. A. (2021). Prospects for the use of shungite as a formaldehyde sorbent in composite materials. Sorbtsionnye I Khromatograficheskie Protsessy, 21(1), pp. 100-110. (In Russ.) https://doi.org/10.17308/sorpchrom.2021.21/3225Mambwe M., Kalebaila K.K., Johnson T. (2021). Remediation technologies for oil contaminated soil. Global Journal of Environmental Science and Management, 7(3), pp. 1-20. https://doi.org/10.22034/gjesm.2021.3.09Pineda-Flores G., Boll-Argüello G., Lira-Galeana C. et al. (2004). A microbial consortium isolated from a crude oil sample that uses asphaltenes as a carbon and energy source. Biodegradation, 15, pp. 145-151. https://doi.org/10.1023/B:BIOD.0000026476.03744.bbOkunev R., Smirnova E., Giniyatullin K. (2019). Study of the effect of labile organic matter removal from pyrochars on the substrate-induced respiration. SGEM, 19(3.2), pp. 459-465.Qin G., Gong D., Fan M.Y. (2013). Bioremediation of petroleumcontaminated soil by biostimulation amended with biochar. International Biodeterioration and Biodegradation, 85, pp. 150-155. https://doi.org/10.1016/j.ibiod.2013.07.004Pineda-Flores G., Boll-Arguello G., Lira-Galeana C., Mesta-Howard A.M. (2004). A microbial consortium isolated from a crude oil sample that uses asphaltenes as a carbon and energy source. Biodegradation, 15(3), pp. 145-151. https://doi.org/10.1023/b:biod.0000026476.03744.bbSharma A., Rehman M.B. (2009). Laboratory scale bioremediation of diesel hydrocarbon in soil by indigenous bacterial consortium. Indian Journal of Experimental Biology, 47(9), pp. 766-9.Pineda-Flores G., Boll-Argüello G., Lira-Galeana C. et al. (2004). A microbial consortium isolated from a crude oil sample that uses asphaltenes as a carbon and energy source. Biodegradation, 15, pp. 145-151. https://doi.org/10.1023/B:BIOD.0000026476.03744.bbSineva A.V., Parfenova A.M., Fedorova A.A. (2007). Adsorption of micelle forming and non-micelle forming surfactants on the adsorbents of different nature. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 306(1-3), pp. 68-74. https://doi.org/10.1016/j.colsurfa.2007.04.061Qin G., Gong D., Fan M.Y. (2013). Bioremediation of petroleumcontaminated soil by biostimulation amended with biochar. International Biodeterioration and Biodegradation, 85, pp. 150-155. https://doi.org/10.1016/j.ibiod.2013.07.004Skrypnik L., Babich O., Sukhikh S. et al. (2021). A Study of the Antioxidant, Cytotoxic Activity and Adsorption Properties of Karelian Shungite by Physicochemical Methods. Antioxidants, 10(7), 1121. https://www.mdpi.com/2076-3921/10/7/1121Sharma A., Rehman M.B. (2009). Laboratory scale bioremediation of diesel hydrocarbon in soil by indigenous bacterial consortium. Indian Journal of Experimental Biology, 47(9), pp. 766-9.Smirnova E.V., Giniyatullin K.G., Okunev R.V. et al. (2016). The Effect of Pre-Incubation Duration of Soil-Biochar Model Mixtures On the Results of Determination the Intensity of Substrate-Induced Respiration (Methodological Aspects of Study). Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(5), pp. 1360-1366.Sineva A.V., Parfenova A.M., Fedorova A.A. (2007). Adsorption of micelle forming and non-micelle forming surfactants on the adsorbents of different nature. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 306(1-3), pp. 68-74. https://doi.org/10.1016/j.colsurfa.2007.04.061Smirnova E.V., Giniyatullin К.G., Valeeva А.А., Vaganova Е.S. (2018). Pyrochars as promising soil ameliorants: Assessment of content and spectral properties of their lipid fractions. Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki, (160)2, pp. 259-275.Skrypnik L., Babich O., Sukhikh S. et al. (2021). A Study of the Antioxidant, Cytotoxic Activity and Adsorption Properties of Karelian Shungite by Physicochemical Methods. Antioxidants, 10(7), 1121. https://www.mdpi.com/2076-3921/10/7/1121Solntseva N.P. (1998). Oil production and geochemistry of natural landscapes. Moscow: Moscow University Press, 376 p. (In Russ.)Smirnova E.V., Giniyatullin K.G., Okunev R.V. et al. (2016). The Effect of Pre-Incubation Duration of Soil-Biochar Model Mixtures On the Results of Determination the Intensity of Substrate-Induced Respiration (Methodological Aspects of Study). Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(5), pp. 1360-1366.Solov’eva A.B., Rozhkova N.N., Glagolev N.N. et al. (1999). Organic matter in schungite and its physicochemical activity in polymeric composites. Russian Journal of Physical Chemistry A, 73(2), pp. 299-303.Solov’eva A.B., Rozhkova N.N., Glagolev N.N. et al. (1999). Organic matter in schungite and its physicochemical activity in polymeric composites. Russian Journal of Physical Chemistry A, 73(2), pp. 299-303.Valeeva A.A., Grigoryan B.R., Bayan M.R. et al. (2015). Adsorption of Methylene Blue by Biochar Produced Through Torrefaction and Slow Pyrolysis from Switchgrass. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(4), pp. 8-17.Valeeva A.A., Grigoryan B.R., Bayan M.R. et al. (2015). Adsorption of Methylene Blue by Biochar Produced Through Torrefaction and Slow Pyrolysis from Switchgrass. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(4), pp. 8-17.Varjani, S. J. (2017). Microbial degradation of petroleum hydrocarbons. Bioresource Technology, 223, pp. 277–286. https://doi.org/10.1016/j.biortech.2016.10.037Varjani, S. J. (2017). Microbial degradation of petroleum hydrocarbons. Bioresource Technology, 223, pp. 277–286. https://doi.org/10.1016/j.biortech.2016.10.037Xu X., Liu W., Tian S. et al. (2018). Petroleum Hydrocarbon-Degrading Bacteria for the Remediation of Oil Pollution Under Aerobic Conditions: A Perspective Analysis. Frontiers in Microbiology, 9, 2885. https://doi.org/10.3389/fmicb.2018.02885Xu X., Liu W., Tian S. et al. (2018). Petroleum Hydrocarbon-Degrading Bacteria for the Remediation of Oil Pollution Under Aerobic Conditions: A Perspective Analysis. Frontiers in Microbiology, 9, 2885. https://doi.org/10.3389/fmicb.2018.02885Yadykina V.V., Vyrodova K.S., Potapov E.E. (2020). Efficiency of using shungite filler for modifying organic binder. IOP Conference. Series: Materials Science and Engineering, 945, 012025. https://doi.org/10.1088/1757-899X/945/1/012025Yadykina V.V., Vyrodova K.S., Potapov E.E. (2020). Efficiency of using shungite filler for modifying organic binder. IOP Conference. Series: Materials Science and Engineering, 945, 012025. https://doi.org/10.1088/1757-899X/945/1/012025Yelikbayev B., Mussina U., Jamalova G. et al. (2017). Bioremedation of oil-polluted soils based on natural and technogenic carbon-containing bioactivator – Koksu shungite. Experimental Biology, 73, pp. 141-152. https://doi.org/10.26577/EB-2017-4-1309Yelikbayev B., Mussina U., Jamalova G. et al. (2017). Bioremedation of oil-polluted soils based on natural and technogenic carbon-containing bioactivator – Koksu shungite. Experimental Biology, 73, pp. 141-152. https://doi.org/10.26577/EB-2017-4-1309Zahed M.A., Salehi S., Madadi R., Hejab F. (2021). Biochar as a sustainable product for remediation of petroleum contaminated soil. Current Research in Green and Sustainable Chemistry, 4, 100055. https://doi.org/10.1016/j.crgsc.2021.100055Zahed M.A., Salehi S., Madadi R., Hejab F. (2021). Biochar as a sustainable product for remediation of petroleum contaminated soil. Current Research in Green and Sustainable Chemistry, 4, 100055. https://doi.org/10.1016/j.crgsc.2021.100055Zargar A.N., Kumar A., Sinha A. et al. (2021). Asphaltene biotransformation for heavy oil upgradation. AMB Express, 11, 127. https://doi.org/10.1186/s13568-021-01285-7Zargar A.N., Kumar A., Sinha A. et al. (2021). Asphaltene biotransformation for heavy oil upgradation. AMB Express, 11, 127. https://doi.org/10.1186/s13568-021-01285-7Zhang C., Wu D., Ren H. (2020). Bioremediation of oil contaminated soil using agricultural wastes via microbial consortium. Scientific Reports, 10, art. 9188. https://doi.org/10.1038/s41598-020-66169-5Zhang C., Wu D., Ren H. (2020). Bioremediation of oil contaminated soil using agricultural wastes via microbial consortium. Scientific Reports, 10, art. 9188. https://doi.org/10.1038/s41598-020-66169-5Zhang H., Tang J., Wang L., et al. (2016). A novel bioremediation strategy for petroleum hydrocarbon pollutants using salt tolerant Corynebacterium variabile HRJ4 and biochar. Journal of Environmental Sciences, 47, pp. 7-13. https://doi.org/10.1016/j.jes.2015.12.023Zhang H., Tang J., Wang L., et al. (2016). A novel bioremediation strategy for petroleum hydrocarbon pollutants using salt tolerant Corynebacterium variabile HRJ4 and biochar. Journal of Environmental Sciences, 47, pp. 7-13. https://doi.org/10.1016/j.jes.2015.12.023Zhuravleva A., Labutova N., Andronov E. (2018). Influence of oil pollution on the microbiocenosis of soils adjacent to the oil storage. Ecological genetics, 15, pp. 60-68. (In Russ.) https://doi.org/10.17816/ecogen15460-68Zhuravleva A., Labutova N., Andronov E. (2018). Influence of oil pollution on the microbiocenosis of soils adjacent to the oil storage. Ecological genetics, 15, pp. 60-68. https://doi.org/10.17816/ecogen15460-68Zhuravleva A., Labutova N., Andronov E. (2018). Influence of oil pollution on the microbiocenosis of soils adjacent to the oil storage. Ecological genetics, 15, pp. 60-68. https://doi.org/10.17816/ecogen15460-68

The authors declare that there are no conflicts of interest present.